Difference between revisions of "Part:BBa K2286011"

 
 
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<partinfo>BBa_K2286011 short</partinfo>
 
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This is the site-directed mutant of halohydrin dehalogenase. Based on the excellent mutant HheC-W249P, We change the phenylalanine to glutamine in the 12 site and phenylalanine to leucine in the 186 site. And this sequence is codon-optimized for E.coli.
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This is the site-directed mutant of halohydrin dehalogenase. Based on the excellent mutant HheC-W249P, We change the phenylalanine  
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to glutamine in the 12 site and phenylalanine to leucine in the 186 site. And this sequence is codon-optimized for E.coli.
  
 
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K2286011 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2286011 SequenceAndFeatures</partinfo>
  

Latest revision as of 13:11, 20 October 2017


HheC-F12Q-F186L-W249P: halohydrin dehalogenase site-directed mutant

This is the site-directed mutant of halohydrin dehalogenase. Based on the excellent mutant HheC-W249P, We change the phenylalanine to glutamine in the 12 site and phenylalanine to leucine in the 186 site. And this sequence is codon-optimized for E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]