Difference between revisions of "Part:BBa K2286007"

 
 
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<partinfo>BBa_K2286007 short</partinfo>
 
<partinfo>BBa_K2286007 short</partinfo>
  
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This is the site-directed mutant of halohydrin dehalogenase. We change the phenylalanine to glutamine in the 12 site and phenylalanine to leucine in the 186 site. And this sequence is codon-optimized for E.coli.
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This is the site-directed mutant of halohydrin dehalogenase. We change the phenylalanine to glutamine in the 12 site and phenylalanine  
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to leucine in the 186 site. And this sequence is codon-optimized for E.coli.
  
 
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Latest revision as of 12:59, 20 October 2017


HheC- F12Q-F186L: halohydrin dehalogenase site-directed mutant

This is the site-directed mutant of halohydrin dehalogenase. We change the phenylalanine to glutamine in the 12 site and phenylalanine to leucine in the 186 site. And this sequence is codon-optimized for E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 718
  • 1000
    COMPATIBLE WITH RFC[1000]