Difference between revisions of "Part:BBa K2368005"
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<partinfo>BBa_K2368005 short</partinfo> | <partinfo>BBa_K2368005 short</partinfo> | ||
| − | <P><i>Pfus</i> is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in <i>S.cerevisiae</i>,oblique.</P> when STE2 binding with α pheromone, STE12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast. | + | <P><i>Pfus</i> is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in <i>S.cerevisiae</i>,oblique.</P> when STE2 binding with α pheromone, STE12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast. |
<P>However, the sequence of <i>Pfus</i> contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P> | <P>However, the sequence of <i>Pfus</i> contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P> | ||
<P>https://static.igem.org/mediawiki/parts/1/11/T_BIT-China_2017part_K2368005.jpeg</P> | <P>https://static.igem.org/mediawiki/parts/1/11/T_BIT-China_2017part_K2368005.jpeg</P> | ||
| − | + | <P>fig.1 The sequencing result of <i>Pfus</i></P> | |
| + | <P>This part is an improvement of BBa_K775004 of 2013 SCUT.</P> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:38, 20 October 2017
Introduction
Pfus
Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae,oblique.
when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)
fig.1 The sequencing result of Pfus
This part is an improvement of BBa_K775004 of 2013 SCUT.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]