Revision as of 11:35, 20 October 2017

Introduction

P_fus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae<p style=“font-style:oblique">S.cerevisiae,oblique.

when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast. </P>

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 <partinfo>BBa_K2368005 short</partinfo>
-<P>Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae<p style=“font-style:oblique">S.cerevisiae,oblique.</P> when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.  </P>
+<P>Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in <i>S.cerevisiae</i><p style=“font-style:oblique"><i>S.cerevisiae</i>,oblique.</P> when STE2 binding with α pheromone, STE12 will stimulate <i>FUS1</i> promoter, thereby initiating the mating of yeast.  </P>
-<P>However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
+<P>However, the sequence of <i>Pfus</i> contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
 <P>https://static.igem.org/mediawiki/parts/1/11/T_BIT-China_2017part_K2368005.jpeg</P>

Difference between revisions of "Part:BBa K2368005"

Revision as of 11:35, 20 October 2017

Introduction

Sequence and Features