Difference between revisions of "Part:BBa K2201411:Design"

 
(→‎Design Notes)
 
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===Design Notes===
 
===Design Notes===
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ttttaaaaaactaacagttgtcagcctgtcccgctttaatatcatacgccgttatacgttgtttacgctttgaggaatcccatatggacgaatttgaaatgataaagagaaacacatctgaaattatcagcgaggaagagttaagagaggttttaaaaaaagatgaaaaatctgctcgtataggttttgaaccaagtggtaaaatacatttagggcattatctccaaataaaaaagatgattgatttacaaaatgctggatttgatataattatattgttggctgatttactggcctatttaaaccagaaaggagagttggatgagattagaaaaataggagattataacaaaaaagtttttgaagcaatggggttaaaggcaaaatatgtttatggaagtgagttccagcttgataaggattatacactgaatgtctatagattggctttaaaaactaccttaaaaagagcaagaaggagtatggaacttatagcaagagaggatgaaaatccaaaggttgctgaagttatctatccaataatgatggttaatggtctgcattatcatggcgttgatgttgctgttggagggatggagcagagaaaaatacacatgttagcaagggagcttttaccaaaaaaggttgtttgtattcacaaccctgtcttaacgggtttggatggagaaggaaagatgagttcttcaaaagggaattttatagctgttgatgactctccagaagagattagggctaagataaagaaagcatactgcccagctggagttgttgaaggaaatccaataatggagatagctaaatacttccttgaatatcctttaaccataaaaaggccagaaaaatttggtggagatttgacagttaatagctatgaggagttagagagtttatttaaaaataaggaattgcatccaatgcgcttaaaaaatgctgtagctgaagaacttataaagattttagagccaattagaaagagattataa
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We recommend to not clone the synthetase in combination with the tRNA on the library plasmid, if it is used for the selection of an orthogaonal amino acyl synthetase/tRNA (aaRS/tRNA)
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===Source===
 
===Source===

Latest revision as of 10:04, 20 October 2017


Tyrosyl synthetase library plasmid from M. jannashii with mRFP as optical controle for a selection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1275
    Illegal AgeI site found at 1387
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We recommend to not clone the synthetase in combination with the tRNA on the library plasmid, if it is used for the selection of an orthogaonal amino acyl synthetase/tRNA (aaRS/tRNA)

Source

BBa_J04450 BBa_K2201400

References