Difference between revisions of "Part:BBa K2368026"
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<p>The original Pfus (BBa_K1154001) is 201bp long with three STE12 binding sites, and the STE12 is Pfus’s transcriptional activator in the endogenous GPCR pathways of yeast.</p>
 
<p>The original Pfus (BBa_K1154001) is 201bp long with three STE12 binding sites, and the STE12 is Pfus’s transcriptional activator in the endogenous GPCR pathways of yeast.</p>
 
<p><i>这里的字体是斜体</i></p>
 
<p><i>这里的字体是斜体</i></p>
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https://static.igem.org/mediawiki/parts/5/5d/T_BIT-China_2017part_K2368026.png
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<img src="" style="width: 100%" />
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<img src="https://static.igem.org/mediawiki/parts/5/5d/T_BIT-China_2017part_K2368026.png" style="width: 100%" />
 
<p style="text-align: center">fig.1 The sequence of original Pfus</p>
 
<p style="text-align: center">fig.1 The sequence of original Pfus</p>
 
<p>In order to enhance the transcription initiation activity of the promoter, we add additional one binding site(gatgaaacaa) in the front of the promoter.</p>
 
<p>In order to enhance the transcription initiation activity of the promoter, we add additional one binding site(gatgaaacaa) in the front of the promoter.</p>
 
<p>And we add 5umol/L α pheromone to detect the modified promoter, and the result is shown in fig.2.</p>
 
<p>And we add 5umol/L α pheromone to detect the modified promoter, and the result is shown in fig.2.</p>
 
<p>http://prts.igem.org/wiki/images/c/ca/T_BIT-China_2017part2_K2368026.png</p>
 
<p>http://prts.igem.org/wiki/images/c/ca/T_BIT-China_2017part2_K2368026.png</p>
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<p>fig.2 Sequencing result of original Pfus and mutant Pfus</p>
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<p style="text-align: center">fig.2 Sequencing result of original Pfus and mutant Pfus</p>
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<p>After the modified promoter assembling into the detection circuit(加个链接), we added 5umol/L α p
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<p>After the modified promoter assembling into the detection circuit(加个链接), we added 5umol/L α </p>

Revision as of 08:24, 20 October 2017

Introduction

Pfus(4 Ste12 binding sites)

General

The original Pfus (BBa_K1154001) is 201bp long with three STE12 binding sites, and the STE12 is Pfus’s transcriptional activator in the endogenous GPCR pathways of yeast.

这里的字体是斜体

<img src="T_BIT-China_2017part_K2368026.png" style="width: 100%" />

fig.1 The sequence of original Pfus

In order to enhance the transcription initiation activity of the promoter, we add additional one binding site(gatgaaacaa) in the front of the promoter.

And we add 5umol/L α pheromone to detect the modified promoter, and the result is shown in fig.2.

http://prts.igem.org/wiki/images/c/ca/T_BIT-China_2017part2_K2368026.png

fig.2 Sequencing result of original Pfus and mutant Pfus

After the modified promoter assembling into the detection circuit(加个链接), we added 5umol/L α