Difference between revisions of "Part:BBa K2368026"
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Revision as of 08:12, 20 October 2017
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Introduction
Pfus(4 Ste12 binding sites)
General
The original Pfus (BBa_K1154001) is 201bp long with three STE12 binding sites, and the STE12 is Pfus’s transcriptional activator in the endogenous GPCR pathways of yeast.
以<i>标签包裹的字体是斜体
<img class="img-auto-width" src="
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fig.1 The sequence of original Pfus
In order to enhance the transcription initiation activity of the promoter, we add additional one binding site(gatgaaacaa) in the front of the promoter.
And we add 5umol/L α pheromone to detect the modified promoter, and the result is shown in fig.2.
http://prts.igem.org/wiki/images/c/ca/T_BIT-China_2017part2_K2368026.png
fig.2 Sequencing result of original Pfus and mutant Pfus
After the modified promoter assembling into the detection circuit(加个链接), we added 5umol/L α p