Difference between revisions of "Part:BBa K2387003:Experience"
 
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eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls.
 
eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls.
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Used protocol can be found at http://2017.igem.org/Team:Wageningen_UR/Notebook/Protocols
 
Used protocol can be found at http://2017.igem.org/Team:Wageningen_UR/Notebook/Protocols

Latest revision as of 07:48, 20 October 2017


This construct is used to determine the functionality of the araC/pBAD promoter. Furthermore it can be used as a positive control in many fluorescence based experiments.

eYFP was expressed induced with different L-arabinose concentrations (0, 0.0002. 0.002 and 0.2%). Furthermore, sole eYFP termini were used as negative controls.

Used protocol can be found at http://2017.igem.org/Team:Wageningen_UR/Notebook/Protocols 

                       T--Wageningen_UR--araCpBAD_test.png"

We clearly show that increasing the concentration of L-arabinose leads to a higher fluorescent signal due to increased protein production.

Applications of BBa_K2387003

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UNIQ1b3f25cd23c9f143-partinfo-00000000-QINU UNIQ1b3f25cd23c9f143-partinfo-00000001-QINU