Difference between revisions of "Part:BBa K2209001"
(→‎Characterization of part BBa_K2209001)
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=== Characterization of part BBa_K2209001 ===
 
=== Characterization of part BBa_K2209001 ===
 
Result showed as follows with well expression of the dioxygenase which have the potential of different degradation spectrum.  
 
Result showed as follows with well expression of the dioxygenase which have the potential of different degradation spectrum.  
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[[Image:pic 2.png|500px|left|thumb|]]
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Lane M1: Protein marker
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Lane M2: Western blot marker
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Lane PC1: BSA (1 μg)
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Lane PC2: BSA (2 μg)
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Lane NC: Cell lysate without induction
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Lane 1: Cell lysate with induction for 16 h at 15 °C
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Lane 2: Cell lysate with induction for 4 h at 37 °C
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Lane NC1: Supernatant of cell lysate without induction
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Lane NC2: Pellet of cell lysate without induction
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Lane 3: Supernatant of cell lysate with induction for 16 h at 15 °C
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Lane 4: Pellet of cell lysate with induction for 16 h at 15 °C
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Lane 5: Supernatant of cell lysate with induction for 4 h at 37 °C
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Lane 6: Pellet of cell lysate with induction for 4 h at 37 °C
  
  

Revision as of 07:19, 20 October 2017


Q gene of aniline dioxygenase from environmental samples

Q gene is the most important gene in aniline dioxygenase which had been confirmed relating with the degradation spectrum. Bacterial strain from different environment might have different degradation spectrum, compared with previous obtained strain, the combined gene (modified promoter and amplified Q gene) were expressed well.


Characterization of part BBa_K2209001

Result showed as follows with well expression of the dioxygenase which have the potential of different degradation spectrum.

Pic 2.png

Lane M1: Protein marker Lane M2: Western blot marker Lane PC1: BSA (1 μg) Lane PC2: BSA (2 μg) Lane NC: Cell lysate without induction Lane 1: Cell lysate with induction for 16 h at 15 °C Lane 2: Cell lysate with induction for 4 h at 37 °C Lane NC1: Supernatant of cell lysate without induction Lane NC2: Pellet of cell lysate without induction Lane 3: Supernatant of cell lysate with induction for 16 h at 15 °C Lane 4: Pellet of cell lysate with induction for 16 h at 15 °C Lane 5: Supernatant of cell lysate with induction for 4 h at 37 °C Lane 6: Pellet of cell lysate with induction for 4 h at 37 °C


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]