Difference between revisions of "Part:BBa K2209000"

 
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===Usage and Biology===
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===Characterization===
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It is expected that aniline dioxygenase from environment samples could constitutive expression by the modification. Experiment result showed that it is achieved as follows, the gene was expressed whether it is induced.
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Revision as of 07:07, 20 October 2017


Modified promoter of aniline dioxygenase

The regulating protein coding by R gene of aniline dioxygenase can bind to the promoter which inhibiting the transcription and expression of aniline dioxygenase. The key sequences of the promoter could be random mutated which interfering the binding of regulating protein and stimulating transcription and expression of the gene. Two base of the nucleotide in promoter were mutated in this study.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]