Difference between revisions of "Part:BBa K2250000"

Revision as of 04:21, 20 October 2017

This is the coding sequence of RafD, a invertase which can catalyze sucrose to glucose and fructose.

It is the coding sequence of RafD, a invertase which can catalyze the hydrolysis of sucrose to glucose and fructose so that the bacteria can use sucrose in the medium. We link this protein before a HlyA tag to form part BBa_K2250002 so that it can be secreted outside the cell, which can make not only the producer use sucrose, but also other bacteria which can not synthesize this protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1356
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 97
Illegal NgoMIV site found at 968
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 799

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2250000 short</partinfo>
-It is the coding sequence of RafD, a invertase which can catalyze the hydrolysis of sucrose to glucose and fructose so that the bacterial can use sucrose in the medium. We link this protein before a HlyA tag to form part BBa_K2250002 so that it can be secreted outside the cell, which can make not only the producer use sucrose, but also other bacteria which can not synthesize this protein.
+It is the coding sequence of RafD, a invertase which can catalyze the hydrolysis of sucrose to glucose and fructose so that the bacteria can use sucrose in the medium. We link this protein before a HlyA tag to form part BBa_K2250002 so that it can be secreted outside the cell, which can make not only the producer use sucrose, but also other bacteria which can not synthesize this protein.
 <!-- Add more about the biology of this part here