Difference between revisions of "Part:BBa K2302003:Design"
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===References=== | ===References=== | ||
| + | [1]Francisco JA,Earhart CF,Georgiou G.Transport and anchoring of beta-lactamase to the external surface of Escherichia coli.Proc Natl Acad Sci USA.[J],1992,89(7):2713-2717. | ||
Latest revision as of 02:51, 20 October 2017
Lpp-ompA,a peptide that can anchor its fused gene product to the outer membrane.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 433
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the peptide is widely used to express various proteins to be anchored on bacterial membrane, such as GFP and fatty acid binding protein.
Source
it is a synthetic coding sequence based on the biased codons of E. coli.
References
[1]Francisco JA,Earhart CF,Georgiou G.Transport and anchoring of beta-lactamase to the external surface of Escherichia coli.Proc Natl Acad Sci USA.[J],1992,89(7):2713-2717.