Difference between revisions of "Part:BBa K2206011"

Revision as of 23:37, 19 October 2017

Anti-miRNA for hsa-miR-27b-3p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch.

The trigger RNA sequence for BBa_K2206010 is divided among hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
-The trigger sequence can be split into different RNA molecules. Colocalization of the RNA molecules containing the split trigger parts results in a complete trigger RNA sequence that is capable of activating the toehold switch.
+The trigger sequence can be split into different RNA molecules. Colocalization of the split trigger parts results in a complete trigger RNA that is capable of activating the toehold switch.
 The trigger RNA sequence for BBa_K2206010 is divided among hsa-miR-27b-3p and an anti-miRNA. This part is the anti-miRNA required for complete trigger RNA formation and activation of BBa_K2206010. This part contains two hybridisation domains: one that is complementary to the 3' end of hsa-miR-27b-3p and one which is complimentary to the 5' end of BBa_K2206010.