Difference between revisions of "Part:BBa K2255000:Design"

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===Design Notes===
 
===Design Notes===
  
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The Enoyl-CoA hydratase DspI <ref>Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).</ref> is necessary to the production of 2-cis-decenenoic acid by ''Pseudomonas aeruginosa'', so we search its DNA sequence in the genome to create our first biobrick. This enzyme gene is on a operon along the other gene crucial for the formation of 2-cis-decenenoic acid.
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The Enoyl-CoA hydratase DspI<ref>Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).</ref> is necessary to the production of 2-cis-decenenoic acid by ''Pseudomonas aeruginosa'', so we search its DNA sequence in the genome to create our first biobrick. This enzyme gene is on a operon along the other gene crucial for the formation of 2-cis-decenenoic acid.
  
 
We took the gene coding for the right enzyme and optimized its sequence for a production in ''E.coli''. Then a [https://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] prefix and sufix was added.
 
We took the gene coding for the right enzyme and optimized its sequence for a production in ''E.coli''. Then a [https://parts.igem.org/Help:Standards/Assembly/RFC10 Rfc10] prefix and sufix was added.

Revision as of 15:09, 19 October 2017


Enoyl-CoA hydratase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1093
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

The Enoyl-CoA hydratase DspI[1] is necessary to the production of 2-cis-decenenoic acid by Pseudomonas aeruginosa, so we search its DNA sequence in the genome to create our first biobrick. This enzyme gene is on a operon along the other gene crucial for the formation of 2-cis-decenenoic acid.

We took the gene coding for the right enzyme and optimized its sequence for a production in E.coli. Then a Rfc10 prefix and sufix was added.

Source

It comes from the genome of Pseudomonas aeruginosa.

References

  1. ↑ Amari, D. T., Marques, C. N. H. & Davies, D. G. The Putative Enoyl-Coenzyme A Hydratase DspI Is Required for Production of the Pseudomonas aeruginosa Biofilm Dispersion Autoinducer cis-2-Decenoic Acid. J. Bacteriol. 195, 4600–4610 (2013).