Difference between revisions of "Part:BBa K2206001"
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Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
 
Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.
  
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This part codes for a toehold switch that contains a region that is complementary to the micro-RNA hsa-mir-27b-3p (the trigger RNA). In the presence of hsa-mir-27b-3p, translation of the downstream protein coding region occurs.
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This part codes for a toehold switch that contains a region that is complementary to the microRNA hsa-miR-27b-3p (the trigger RNA). In the presence of hsa-miR-27b-3p, translation of the downstream protein coding region occurs.
  
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This part can be used to regulate the expression of any protein in response to the levels of hsa-mir-27b-3p present in a system.
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This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-27b-3p present in a system.
  
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We believe that the transcripts produced by this part are toxic when expressed at reasonably high concentrations in E.coli (e.g. the concentrations produced from the BBa_J23111 constitutive promoter).
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The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found BBa_K808000 was too leaky).
  
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N.B. This part contains a strong RBS sequence.
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This part contains a strong RBS sequence.
  
 
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Revision as of 13:41, 19 October 2017

Toehold switch for hsa-miR-27b-3p

Toehold switches are synthetic riboregulators that regulate gene expression post-transcriptionally. Gene expression can be activated in the presence of a cognate single stranded RNA molecule that contains an arbitrary sequence (the trigger RNA). The trigger RNA binds to the switch through base pairing, causing a conformational change that results in translation of the downstream protein coding region.

This part codes for a toehold switch that contains a region that is complementary to the microRNA hsa-miR-27b-3p (the trigger RNA). In the presence of hsa-miR-27b-3p, translation of the downstream protein coding region occurs.

This part can be used to regulate the expression of any protein in response to the levels of hsa-miR-27b-3p present in a system.

The transcripts produced by this part are toxic to E. coli. We recommend the use of a non-leaky promoter (for reference, we found BBa_K808000 was too leaky).

This part contains a strong RBS sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]