Difference between revisions of "Part:BBa J100337:Design"

 
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===References===
 
===References===
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Orth P, Schnappinger D, Hillen W, Saenger W, and Hinrichs W (2000) Structural Basis of Gene Regulation by the Tetracycline Inducible Tet Repressor–Operator System. 7(3): 215-219, in Nature Structural Biology. www.bio.davidson.edu/113/weekly_Labs/Orthetal_Structure_2000.pdf.
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Ramos JL, Martinez-Bueno M, Molina-Henares AJ, Teran W, Watanabe K, Zhange X, Gallegos MT, Brennan R, Tobes R (2005) The TetR Family of Transcriptional Repressors. 69(2): 326-356 in Microbiology and Molecular Biology Reviews. www.bio.davidson.edu/113/weekly_Labs/Ramosetal_TetRFamily_2005.pdf.

Revision as of 13:12, 19 October 2017


TetR Repressible Promoter from Ramos et al. and Orth et al.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutations made to -7,0, and 7 bases of the TetR binding sites.


Source

Originally comes from E. Coli genome.

References

Orth P, Schnappinger D, Hillen W, Saenger W, and Hinrichs W (2000) Structural Basis of Gene Regulation by the Tetracycline Inducible Tet Repressor–Operator System. 7(3): 215-219, in Nature Structural Biology. www.bio.davidson.edu/113/weekly_Labs/Orthetal_Structure_2000.pdf. Ramos JL, Martinez-Bueno M, Molina-Henares AJ, Teran W, Watanabe K, Zhange X, Gallegos MT, Brennan R, Tobes R (2005) The TetR Family of Transcriptional Repressors. 69(2): 326-356 in Microbiology and Molecular Biology Reviews. www.bio.davidson.edu/113/weekly_Labs/Ramosetal_TetRFamily_2005.pdf.