Difference between revisions of "Part:BBa K2443010"

Revision as of 14:47, 18 October 2017

IleRS optimized for expression in E. coli

isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1419
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 640
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 147
Illegal SapI.rc site found at 1538

Revision as of 14:47, 18 October 2017 (view source) Registry (Talk \| contribs)		Revision as of 14:47, 18 October 2017 (view source) Graeme.glaister (Talk \| contribs) Newer edit →
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	<partinfo>BBa_K2443010 short</partinfo>		<partinfo>BBa_K2443010 short</partinfo>

−	isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.	+	isoleucine tRNA synthetase responsible for attaching isoleucine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.