Difference between revisions of "Part:BBa K2387046"
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<partinfo>BBa_K2387046 short</partinfo> | <partinfo>BBa_K2387046 short</partinfo> | ||
| − | This a split version of BBa_K2387045. The two halves of mVenus are fused to leucine zippers to induce the reassembly. Both parts are under the control of the araC/pBad promoter and each part is under the control of a stron RBS. The reassembly process is irreversible. This part can be used as a positive control for experiments using BiFC, as well as a source for the fragments of mVenus. | + | This a split version of [https://parts.igem.org/cgi/partsdb/add_part_c.cgi BBa_K2387045]. The two halves of mVenus are fused to leucine zippers to induce the reassembly. Both parts are under the control of the araC/pBad promoter and each part is under the control of a stron RBS. The reassembly process is irreversible. This part can be used as a positive control for experiments using BiFC, as well as a source for the fragments of mVenus. |
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Revision as of 10:09, 18 October 2017
Split mVenus controlled by inducible araC/pBAD promoter
This a split version of BBa_K2387045. The two halves of mVenus are fused to leucine zippers to induce the reassembly. Both parts are under the control of the araC/pBad promoter and each part is under the control of a stron RBS. The reassembly process is irreversible. This part can be used as a positive control for experiments using BiFC, as well as a source for the fragments of mVenus.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961