Difference between revisions of "Part:BBa K2324003:Design"

 
 
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===Design Notes===
 
===Design Notes===
Codon optimisation for expression in E.coli
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Codon optimisation for expression in <i>E. coli </i>. Research was required to identify the ideal placement of the foreign protein domain.
 
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Latest revision as of 19:12, 16 October 2017


FimH+sfGFP at residue 258


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimisation for expression in E. coli . Research was required to identify the ideal placement of the foreign protein domain.


Source

The FimH sequence is a codon optimised version of the registry entry BBa_K1850001. The sfGFP sequence is a codon optimised version of the registry entry BBa_I746916.

References