Difference between revisions of "Part:BBa K2332010:Design"

 
(→‎Source)
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
Sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences
+
Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences
 
And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html
 
And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html
−
Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001  
+
Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001
 +
SpyTag: BBa_K1159201
  
 
===References===
 
===References===

Revision as of 16:25, 16 October 2017


Intimin'-SpyTag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 801
    Illegal NgoMIV site found at 1542
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to fuse SpyTag to the N-terminal of a truncated version of Intimin as this has been previously proven to function effectively for cell surface display of up to 30kDa passenger proteins


Source

Intimin protein sequence obtained from: http://www.uniprot.org/uniprot/P43261#sequences And reverse translated using: http://www.bioinformatics.org/sms2/rev_trans.html Intimin for the cell surface display of our tag was truncated according to Wentzel et al., 2001 SpyTag: BBa_K1159201

References