Difference between revisions of "Part:BBa K2408023"
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Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag
 
Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag
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The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective
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we added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:59, 15 October 2017


ADH1_Miraculin

Miraculin optimized for S. cerevisae with the ADH1 promoter and histidine tag

The miraculin glycoprotein, originally produced from the Synsepalum dulcificum plant, is an effectively non-cariogenic and acceptable for diabetics in flavor and sweetening formulations and has the ability to convert the sour taste to the sweet taste, and provides a sugar substitute because it has a glycemic index of zero. However, miraculin does not change the taste of the foods / drinks consumed. The mechanism of action of the special protein is still not entirely clear but it is known that in the presence of food / beverage products with a pH level between 3-6 factors trigger the reaction that will give the sense of sweetness effective we added the ADH1 strong promoter at the beginning of the sequence and a histidine tag (his * 6) and terminator at the end.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1110
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1222
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1110
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 93