Difference between revisions of "Part:BBa K2387004:Design"

 
(Design Notes)
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
Double stop codon (TAATAA) added
+
Double stop codon (TAATAA) added after eYFPn
 
+
 
+
  
 
===Source===
 
===Source===

Latest revision as of 11:39, 15 October 2017


eYFP N-terminus controlled by inducible araC/pBAD promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

Double stop codon (TAATAA) added after eYFPn

Source

eYFP originates from A. victoria araC/pBAD promoter originates from E. coli All parts taken from the repository

References