Difference between revisions of "Part:BBa K2269027"

Latest revision as of 10:06, 15 October 2017

Tnos:YFP:Assembly Reporter PB:Luc:Tnos

Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 819
Illegal XbaI site found at 506
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 819
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 819
Illegal BglII site found at 302
Illegal BglII site found at 542
Illegal BglII site found at 649
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 819
Illegal XbaI site found at 506
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 819
Illegal XbaI site found at 506
1000
COMPATIBLE WITH RFC[1000]

Revision as of 10:05, 15 October 2017 (view source) Albagoutiel (Talk \| contribs) ← Older edit		Latest revision as of 10:06, 15 October 2017 (view source) Albagoutiel (Talk \| contribs)
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	<partinfo>BBa_K2269027 short</partinfo>		<partinfo>BBa_K2269027 short</partinfo>

−	Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.	+	Transcriptional unit composed of the 35s inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attP and attB). In normal basis, a YFP will be expressing and only when RDF appears, luciferase will be expressed.