Difference between revisions of "Part:BBa K2269025"

 
 
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<partinfo>BBa_K2269025 short</partinfo>
 
<partinfo>BBa_K2269025 short</partinfo>
  
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Transcriptional unit composed of the PR-1a inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL). In normal basis, a stuffer fragment will be expressing and only when RDF appears, Ros 1 will be expressed.
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Middle part for assembly of site-specific recombination based Registers. Composed of the 35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL).
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 09:32, 15 October 2017


Assembly Reporter RL phiC31 Middle Part

Middle part for assembly of site-specific recombination based Registers. Composed of the 35S promoter inverted sequence and the Mtb terminator flanked by two opposing PhiC31 site-specific recombination sites (attR and attL).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 54
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 54
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 54
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 54
  • 1000
    COMPATIBLE WITH RFC[1000]