Difference between revisions of "Part:BBa K2246010:Design"

 
(Design Notes)
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
BamHI restriction site as result from clonation into BBa_K2246000.
 
BamHI restriction site as result from clonation into BBa_K2246000.
 
+
A single mutation is present at at base 645 from the original BBa_K592009 sequence in order to prevent a HinCII cut site.
 
+
  
 
===Source===
 
===Source===

Latest revision as of 14:58, 14 October 2017


anti-GFP BSLA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 821
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 750
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BamHI restriction site as result from clonation into BBa_K2246000. A single mutation is present at at base 645 from the original BBa_K592009 sequence in order to prevent a HinCII cut site.

Source

Synthetic Part.

References