Difference between revisions of "Part:BBa K2368013"
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<partinfo>BBa_K2368013 short</partinfo>
 
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<p>In order to confirm the expression and location of sweetness receptor, T1R2 and T1R3, we decide to construct the fusion protein, T1R3- amilGFP (K592010). The part K592010 is yellow chromoprotein and can exhibit strong yellow color when expressed. Because the T1R3 is belonged to C-class family G protein coupling receptor (GPCR) and the carboxyl terminal of GPCR interacts with the G protein &#945;subunit ,which is a crucial factor during signal deliver through G protein signal pathway, we perform the fusion between the N-terminal of T1R3 and C-terminal of amilGFP. And hope to show the location of T1R3 through fluorescence microscope for detecting amilGFP.</p>
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<P>This part is that sweetness receptor T1R3 combines with the YCP tag. </P>
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<P>The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R3 and YCP tag.</P>
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<P>The result of the part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3. </P>
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Revision as of 03:15, 14 October 2017


Introduction

T1R3-YCP

This part is that sweetness receptor T1R3 combines with the YCP tag.

The T1R3 belongs to the C-class family of G protein coupling receptor (GPCR). And the C-terminal of GPCR that interacts with the G protein α subunit , which is a crucial factor during signal deliver through G protein signal pathway is so important that it can’t be modified. Also, it is necessary for us to show whether the T1R3 expressed correctly. As a result of that, we can only perform the fusion between the N-terminal of T1R3 and YCP tag.

The result of the part is that the expression of the yellow cyanidin protein to detect the expression and the location of the T1R3.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]