Difference between revisions of "Part:BBa K2368007:Design"
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
This is a complete protein expression box of T1R2.
+
We construct the part by Gibson assembly.And the Gal1/Gal10 promoter and the CYC1 terminator stem from the PESC-Ura plasmid.
  
 
===Source===
 
===Source===

Revision as of 02:46, 14 October 2017


PGal 1/10-T1R2-CYC1t


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 563
    Illegal BglII site found at 2061
    Illegal BglII site found at 2481
    Illegal BglII site found at 2643
    Illegal BamHI site found at 674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 291
    Illegal AgeI site found at 1572
    Illegal AgeI site found at 2790
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We construct the part by Gibson assembly.And the Gal1/Gal10 promoter and the CYC1 terminator stem from the PESC-Ura plasmid.

Source

References