Difference between revisions of "Part:BBa K2200000"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | A well-designed gRNA is able to highly enhance the specificity with Cas9 nickase. The specificity of the CRISPR system is determined in large part by how specific the gRNA targeting sequence is for the genomic target compared to the rest of the genome. | ||
| + | We choose the nickase system for highly specific gene editing. Cas9 nickase binds DNA based on gRNA specificity, only resulting in “nicks”, or single strand breaks, instead of double strand breaks (DSB).DNA nicks are rapidly repaired by homology directed repair (HDR) using the intact complementary DNA strand as the template. | ||
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Revision as of 02:25, 14 October 2017
Guide RNA (gRNA) is a specific molecule that guides the Cas nuclease to a targeted dsDNA sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]