Difference between revisions of "Part:BBa K2368005"
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<partinfo>BBa_K2368005 short</partinfo>
 
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<P>Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae, when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast. </P>
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<P>However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)</P>
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Revision as of 02:05, 14 October 2017


Introduction

Pfus

Pfus is an induced promoter from Saccharomyces cerevisiae. In the endogenous MAPK pathway in S.cerevisiae, when STE2 binding with α pheromone, STE12 will stimulate FUS1 promoter, thereby initiating the mating of yeast.

However, the sequence of Pfus contains Pst1 restrict enzyme site, so we use the method of one-step mutation to achieve the purpose of site-directed mutagenesis.(from CTGCAG to CTACAT)


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]