Difference between revisions of "Part:BBa K2387001"
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===Usage and Biology===
 
===Usage and Biology===
  
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anm2CP is a monomeric chromoprotein (BBa_K2387000), the only one that is known to be produced in nature. The sequence can be retrived from the genome of the jellyfish Anthomedusae sp. SL-2003 (GenBank, AY485336). It shows a bright pink color when expressed to sufficient concentrations. Its absorbance spectrum shows a pick with a maximum at 572 nm. As other fluorescent proteins and chromoproteins, it has a structure similar to that of GFP, as it can be observed from the structure of KillerRed (PSB 2WIQ) a fluorescent protein evolved from anm2CP. Most chromoproteins are tetrameric, which makes them a bad candidate for protein fusions and other applications. Among other applications, anm2CP would be able to be split in order to be used in bimolecular complementation techniques.
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anm2CP is a <b>monomeric</b> chromoprotein (BBa_K2387000), the only one that is known to be produced in nature. The sequence can be retrived from the genome of the jellyfish Anthomedusae sp. SL-2003 (GenBank, AY485336). It shows a bright pink color when expressed to sufficient concentrations. Its absorbance spectrum shows a pick with a maximum at 572 nm. As other fluorescent proteins and chromoproteins, it has a structure similar to that of GFP, as it can be observed from the structure of KillerRed (PSB 2WIQ) a fluorescent protein evolved from anm2CP. Most chromoproteins are tetrameric, which makes them a bad candidate for <b>protein fusions</b> and other applications. Among other applications, anm2CP would be able to be <b>split</b> in order to be used in bimolecular complementation techniques.
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[[file:Anm2CP.jpeg|400px|center|thumb|<p align="justify">'''Figure 1: On the left, E. coli BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.'''</p>]]
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2387001 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2387001 SequenceAndFeatures</partinfo>
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K2387001 parameters</partinfo>
 
<partinfo>BBa_K2387001 parameters</partinfo>

Revision as of 13:31, 13 October 2017


Monomeric chromoprotein anm2CP controlled by inducible araC/pBAD promoter


In this composite, the coding sequence for anm2CP is under the control of the inducible pBad/araC promoter (BBa_I0500), as well as a strong RBS (BBa_B0034).

Usage and Biology

anm2CP is a monomeric chromoprotein (BBa_K2387000), the only one that is known to be produced in nature. The sequence can be retrived from the genome of the jellyfish Anthomedusae sp. SL-2003 (GenBank, AY485336). It shows a bright pink color when expressed to sufficient concentrations. Its absorbance spectrum shows a pick with a maximum at 572 nm. As other fluorescent proteins and chromoproteins, it has a structure similar to that of GFP, as it can be observed from the structure of KillerRed (PSB 2WIQ) a fluorescent protein evolved from anm2CP. Most chromoproteins are tetrameric, which makes them a bad candidate for protein fusions and other applications. Among other applications, anm2CP would be able to be split in order to be used in bimolecular complementation techniques.

Figure 1: On the left, E. coli BL21 without plasmid. On the right, E. coli BL21 expressing anm2CP induced by 0.2% arabinose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1280
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

Functional Parameters