Difference between revisions of "Part:BBa K2328005"

Revision as of 09:28, 13 October 2017

p32 High copy number BioBrick assembly replication

p32 is the replication of the plasmid pMG36e, which is a high copy number plasmid.The strong promoter P32 was discovered in 1987 by VanDer Vossen et al. Which was obtained from the cloning of Streptococcus cholerae by shotgun method. P32 includes an open reading frame and a ribosome binding site which can be recognized by Escherichia coli, Bacillus subtilis and Streptococcus.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 1
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 1
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 1
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 1
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 1
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 p32 is the replication of the plasmid pMG36e, which is a high copy number plasmid.The strong promoter P32 was discovered in 1987 by VanDer Vossen et al. Which was obtained from the cloning of Streptococcus cholerae by shotgun method. P32 includes an open reading frame and a ribosome binding site which can be recognized by Escherichia coli, Bacillus subtilis and Streptococcus.
-====Usage and Biology====
-The p32 promoter can be replicated either in Lactococcus or in E. coli. As a promoter for shuttle plasmids, the p32 promoter can initiate expression of the Smurfp protein in E. coli and Lactococcus.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+The p32 promoter can be replicated either in Lactococcus or in E. coli. As a promoter for shuttle plasmids, the p32 promoter can initiate expression of the Smurfp protein in E. coli and Lactococcus.
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