Difference between revisions of "Part:BBa K2447016:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Test
+
Silent mutations were introduced to be RFC[10] compatible
  
  
  
 
===Source===
 
===Source===
 +
TlpA, a transcriptional autorepressor from the virulence plasmid of Salmonella typhimurium.
  
−
Test
+
http://www.nature.com/nchembio/journal/v13/n1/full/nchembio.2233.html?WT.feed_name=subjects_synthetic-biology
  
 
===References===
 
===References===

Latest revision as of 14:33, 12 October 2017


Phosphate-dependent, temperature-sensitive cascaded system with IM2 expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1026
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2085
    Illegal XhoI site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1614
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Silent mutations were introduced to be RFC[10] compatible


Source

TlpA, a transcriptional autorepressor from the virulence plasmid of Salmonella typhimurium.

http://www.nature.com/nchembio/journal/v13/n1/full/nchembio.2233.html?WT.feed_name=subjects_synthetic-biology

References