Difference between revisions of "Part:BBa K2447016"

 
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<partinfo>BBa_K2447016 short</partinfo>
 
<partinfo>BBa_K2447016 short</partinfo>
  
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Test
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This design of this cascade system is similar to our other part [https://parts.igem.org/wiki/index.php?title=Part:BBa_K2447015 BBa_K2447015], with the only exception where the GFP coding sequence is being replaced by E2 immunity protein.
  
 
<!-- Add more about the biology of this part here
 
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Revision as of 14:18, 12 October 2017


Phosphate-dependent, temperature-sensitive cascaded system with IM2 expression

This design of this cascade system is similar to our other part BBa_K2447015, with the only exception where the GFP coding sequence is being replaced by E2 immunity protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1026
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2085
    Illegal XhoI site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1614
  • 1000
    COMPATIBLE WITH RFC[1000]