Difference between revisions of "Part:BBa K2308004"

Latest revision as of 15:44, 11 October 2017

used to knock out the gene crtB in the genome of Rhodobacter Sphaeroides+ Amp

This part is used to knock out the gene crtB in Rhodobacter Sphaeroides, using the method of redET. The part is composed of 4 basic parts(BBa_K2308006,BBa_K2308010,BBa_K2308011,BBa_K2308007) If the gene crtB is knocked out by homologous recombination, the strain will be selected by Amp resistance.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 93
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 47
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1250
Illegal BsaI.rc site found at 162
Illegal SapI site found at 1759

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2308004 short</partinfo>
-This part is used to knock out the gene crtB in Rhodobacter Sphaeroides. The part is composed of 806bp of upstream DNA sequence and 433bp of downstream DNA sequences, and crtB can be knocked out by homologous recombination. An Amp resistance promoter and Amp resistance gene is added between the up/downstream sequences so that the clones after homologous recombination can be selected by Amp. The upstream sequence is a little long than that of BBa_K2308001 so that the recombination because of the low efficiency of BBa_K2308004.
+This part is used to knock out the gene crtB in Rhodobacter Sphaeroides, using the method of redET.
+The part is composed of 4 basic parts(BBa_K2308006,BBa_K2308010,BBa_K2308011,BBa_K2308007)
+If the gene crtB is knocked out by homologous recombination, the strain will be selected by Amp resistance.
 <!-- Add more about the biology of this part here