Difference between revisions of "Part:BBa K2242233"

 
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<partinfo>BBa_K2242233 short</partinfo>
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"Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hy- drogen sulfide. Because cysteine desulfhydrase activity is not restricted to anaer- obic conditions, expression of cysteine desulfhydrase in a suitable host could result in aerobic precipitation of cadmium as cadmium sulfide. (Wang, C., Lum, A., Ozuna, S., Clark, D., & Keasling, J. (2001). Aerobic sulfide production and cadmium precipitation by Escherichia coli expressing the Treponema denticola cysteine desulfhydrase gene. Applied microbiology and biotechnology, 56(3-4), 425-430.) In our project, we need to use CdS nanoparticles to generate electrons utilizing light energy. So we need to find a way to produce CdS nanoparticles on the membrane of our engineered E.coli. The easiest way is to precipitate those nanoparticles directly, using the sulfide ions the bacteria produce and the cadmium ion we add into the reaction system. We introduce this enzyme to produce sulfide ion in the cytoplasm of our E.coli efficiently.“
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2242233 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2242233 parameters</partinfo>
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Revision as of 14:29, 11 October 2017


Cysteine Desulfhydrase

"Cysteine Desulfhydrase is an aminotransferase that converts cysteine into pyruvate, ammonia, and hy- drogen sulfide. Because cysteine desulfhydrase activity is not restricted to anaer- obic conditions, expression of cysteine desulfhydrase in a suitable host could result in aerobic precipitation of cadmium as cadmium sulfide. (Wang, C., Lum, A., Ozuna, S., Clark, D., & Keasling, J. (2001). Aerobic sulfide production and cadmium precipitation by Escherichia coli expressing the Treponema denticola cysteine desulfhydrase gene. Applied microbiology and biotechnology, 56(3-4), 425-430.) In our project, we need to use CdS nanoparticles to generate electrons utilizing light energy. So we need to find a way to produce CdS nanoparticles on the membrane of our engineered E.coli. The easiest way is to precipitate those nanoparticles directly, using the sulfide ions the bacteria produce and the cadmium ion we add into the reaction system. We introduce this enzyme to produce sulfide ion in the cytoplasm of our E.coli efficiently.“

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 297
  • 1000
    COMPATIBLE WITH RFC[1000]