Difference between revisions of "Part:BBa K2443067"

Latest revision as of 22:58, 10 October 2017

EF-Tu

EF-Tu (elongation factor thermo unstable) is a prokaryotic elongation factor which catalyzes the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome. It is a G-protein, and is involved in the selection and binding of an aa-tRNA to the A-site of the ribosome. As a result of its crucial role in translation, EF-Tu is one of the most abundant and highly conserved proteins in prokaryotes [2][3][4]. Codon optimized for use in Escherichia coli and contains an N-terminal histidine tag.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2443067 short</partinfo>
-EF-Tu (elongation factor thermo unstable) is a prokaryotic elongation factor which catalyzes the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome. It is a G-protein, and is involved in the selection and binding of an aa-tRNA to the A-site of the ribosome. As a result of its crucial role in translation, EF-Tu is one of the most abundant and highly conserved proteins in prokaryotes.[2][3][4]
+EF-Tu (elongation factor thermo unstable) is a prokaryotic elongation factor which catalyzes the binding of an aminoacyl-tRNA (aa-tRNA) to the ribosome. It is a G-protein, and is involved in the selection and binding of an aa-tRNA to the A-site of the ribosome. As a result of its crucial role in translation, EF-Tu is one of the most abundant and highly conserved proteins in prokaryotes [2][3][4]. Codon optimized for use in <i>Escherichia coli</i> and contains an N-terminal histidine tag.
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