Difference between revisions of "Part:BBa K2443062:Design"

 
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===References===
 
===References===
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Histidine tag at C-terminus (6 histidine residues) with 18 amino acid linker sequence: http://pubs.acs.org/doi/pdf/10.1021/bi9926072

Latest revision as of 22:28, 10 October 2017


MTF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 839
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 195
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for use in Escherichia coli.



Source

uniprot.org

References

Histidine tag at C-terminus (6 histidine residues) with 18 amino acid linker sequence: http://pubs.acs.org/doi/pdf/10.1021/bi9926072