Difference between revisions of "Part:BBa K2443056"

 
 
Line 3: Line 3:
 
<partinfo>BBa_K2443056 short</partinfo>
 
<partinfo>BBa_K2443056 short</partinfo>
  
Proline tRNA synthetase is responsible for attaching proline onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal histidine tag with a serine glycine linker.
+
Proline tRNA synthetase is responsible for attaching proline onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker.
  
  

Latest revision as of 13:21, 10 October 2017


Pro RS

Proline tRNA synthetase is responsible for attaching proline onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1279
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 514