Difference between revisions of "Part:BBa K2443048"

Latest revision as of 12:19, 10 October 2017

Gly RS beta

glutamic acid tRNA synthetase responsible for attaching glutamic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1165
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1698
Illegal SapI site found at 55

Revision as of 12:14, 10 October 2017 (view source) Registry (Talk \| contribs)		Latest revision as of 12:19, 10 October 2017 (view source) Graeme.glaister (Talk \| contribs)
Line 3:		Line 3:
	<partinfo>BBa_K2443048 short</partinfo>		<partinfo>BBa_K2443048 short</partinfo>

−	glutamic acid tRNA synthetase responsible for attaching glutamic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a N-terminal histidine tag with a serine glycine linker.	+	glutamic acid tRNA synthetase responsible for attaching glutamic acid onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker.