Difference between revisions of "Part:BBa K2278021"
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DNY-15 AMP with Alpha-Factor Secretion Signal
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__NOTOC__
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<partinfo>BBa_K2278011 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2278011 SequenceAndFeatures</partinfo>
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=='''Introduction'''==
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<html>
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This DNA biobrick was designed in order to produce in <i></i> strain.
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<h3 id="RT"> 1- Biological background </h3>
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 +
 +
Mécanisme
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<figure><p style="text-align:center;"> <img src ="g" width = "600" /> <figcaption> Figure 1: <btitre </b> figure caption</figcaption> </figure>
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<h3 id="RT"> 2- Usage in iGEM projects </h3>
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<p> The BBa_K2278011 cames from the module of the Croc’n cholera project <a href="http://2017.igem.org/Team:INSA-UPS_France">(team INSA-UPS-France 2017)</a>
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It was designed to produce </p>
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<p>The part includes  </p>
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 +
 
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</html>
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 +
 
 +
 
 +
=='''Experiments'''==
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<html>
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<h3 id="RT"> 1- Molecular biology </h3>
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<p>
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The  gene was placed in silico under the control of the p promoter (BBa_R), a strong RBS (BBa_B0034) and a terminator (BBa_B1006). IDT performed the DNA synthesis and delivered the part as gBlock. 
 +
The construct was cloned by conventional ligation into pSB1C3 plasmid and transformed into E. coli Dh5 alpha strain. X transformants were obtained.
 +
</p>
 +
 
 +
<b>Analysis of the restriction map </b>
 +
 
 +
<figure><p style="text-align:center;"><img src="" width = "600"/><figcaption> Figure 2: <b>title  </b> Digested plasmids are electrophoresed through an 0.7% agarose gel. The desired plasmids lengths are in parentheses. pSB1C3 (2029bp the other band correspond to a xxx bp insert)</figcaption></figure>
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<p><b>Sequencing </p></b>
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<figure><p style="text-align:center;"><img src="" width = "500"/><figcaption> Figure 3: <b>Sequencing  of pSB1C3_  </b> 1500 ng of plasmid are sequenced. X oligos were used to perform the sequencing. The obtained sequence were blast on the BBa_K2278011 sequence with the iGEM sequencing online tools. </figcaption></figure>
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The sequencing show a
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<h3 id="RT"> 2- Expression  <i>in vivo</i>  </h3>
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<p><b>sous titre</b></p>
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<p> Protocole </p>
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 +
 
 +
</html>
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=='''Characterization'''==
 +
<html>
 +
 
 +
<h3 id="RT">1- Validation of  </h3>
 +
description
 +
 
 +
<p> <b>manip1 </b> </p>
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 +
Image stylée
 +
 
 +
<figure><p style="text-align:center;"><img src="" width = "500"/><figcaption> Figure  <b>title </b>légende </figcaption></figure>
 +
 
 +
<p>Interprétation  </p>
 +
 
 +
<p> <b>manip2</b> </p>
 +
Image stylée
 +
 
 +
<figure><p style="text-align:center;"><img src="" width = "500"/><figcaption> Figure  <b>plus de figure !  </figcaption></figure>
 +
 
 +
<p>interprétation </p>
 +
 
 +
<p><b>Discussion : </b> </p>
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<p></p>
 +
 
 +
 
 +
 
 +
<h3 id="RT">2. 2ème approche  </h3>
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<figure><p style="text-align:center;"><img src="" width = "500"/><figcaption> Figure <b>Solid results </b>  légende de qualité </figcaption></figure>
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 +
<p>brillante analyse</p>
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 +
<p><b>Discussion : </b> </p>
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<p>des perspectives éclectiques </p>

Revision as of 11:55, 10 October 2017

pTet driven Diacetyl generator (pTet + ALS)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1738
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Introduction

This DNA biobrick was designed in order to produce in strain.

1- Biological background

Mécanisme

Figure 1: figure caption

2- Usage in iGEM projects

The BBa_K2278011 cames from the module of the Croc’n cholera project (team INSA-UPS-France 2017) It was designed to produce

The part includes


Experiments

1- Molecular biology

The gene was placed in silico under the control of the p promoter (BBa_R), a strong RBS (BBa_B0034) and a terminator (BBa_B1006). IDT performed the DNA synthesis and delivered the part as gBlock.  The construct was cloned by conventional ligation into pSB1C3 plasmid and transformed into E. coli Dh5 alpha strain. X transformants were obtained.

Analysis of the restriction map

Figure 2: title Digested plasmids are electrophoresed through an 0.7% agarose gel. The desired plasmids lengths are in parentheses. pSB1C3 (2029bp the other band correspond to a xxx bp insert)

Sequencing

Figure 3: Sequencing of pSB1C3_ 1500 ng of plasmid are sequenced. X oligos were used to perform the sequencing. The obtained sequence were blast on the BBa_K2278011 sequence with the iGEM sequencing online tools.
The sequencing show a

2- Expression in vivo

sous titre

Protocole

Characterization

1- Validation of

description

manip1

Image stylée

Figure title légende

Interprétation

manip2

Image stylée

Figure plus de figure !

interprétation

Discussion :

2. 2ème approche

Figure Solid results légende de qualité

brillante analyse

Discussion :

des perspectives éclectiques