Difference between revisions of "Part:BBa K2255003"
Line 6: Line 6:
  
 
This part was designed with the Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain.
 
This part was designed with the Freiburg ([https://parts.igem.org/Assembly_standard_25 Rfc25]) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain.
 +
 +
===Usage and Biology===
 +
 +
This part is usefull in our to purify the [http://2017.igem.org/Team:Aix-Marseille/DEPS enzyme] that use to disrupt the biofilm.
  
 
==References==
 
==References==
  
 
<references/>
 
<references/>
−
 
−
<!-- Add more about the biology of this part here
 
−
===Usage and Biology===
 
  
 
<!-- -->
 
<!-- -->

Revision as of 09:18, 9 October 2017


Multi-Tag (Rfc 25)

This tag provides multiple fixation sites: a Strep-tag [1] and a histidine tag. Between those tag is a TEV protease cutting site [2].

This part was designed with the Freiburg (Rfc25) extension. Thus, it contains the restriction sites NgoMIV and AgeI that are compatible and allow the absence of a start and stop codon, which ease the assembly of multiple protein domain.

Usage and Biology

This part is usefull in our to purify the [http://2017.igem.org/Team:Aix-Marseille/DEPS enzyme] that use to disrupt the biofilm.

References

  1. ↑ Schmidt, Thomas GM; Skerra, Arne (2007). "The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins". Nature Protocols. 2 (6): 1528–35. PMID 17571060. doi:10.1038/nprot.2007.209
  2. ↑ Parks TD, Leuther KK, Howard ED, Johnston SA, Dougherty WG (February 1994). "Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase". Anal. Biochem. 216 (2): 413–7. PMID 8179197. doi:10.1006/abio.1994.1060

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]