Difference between revisions of "Part:BBa K2206002:Design"
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No terminators are needed as the pSB1C3 plasmid contains terminators. | No terminators are needed as the pSB1C3 plasmid contains terminators. | ||
| − | Between the promoter and the toehold switch there is a scar, | + | Between the promoter and the toehold switch there is a scar, but there is no scar between the toehold switch and the reporter protein. |
===Source=== | ===Source=== | ||
Revision as of 13:29, 8 October 2017
Toehold switch for hsa-miR-15b-5p with GFPmut3b
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 764
Design Notes
No terminators are needed as the pSB1C3 plasmid contains terminators.
Between the promoter and the toehold switch there is a scar, but there is no scar between the toehold switch and the reporter protein.
Source
This part is a synthetic toehold switch that was designed in silico and synthesised by IDT.