Difference between revisions of "Part:BBa K2406053"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | The main goal of our project in the short-term was to develop and test recombinase parts in E. coli. Most of the proteins had not previously been tested in bacteria before. Therefore, the new parts we have developed and tested are recombinases and their target sites. We then confirmed the activity of the recombinase proteins and function of target sites with the composite parts described below. In addition, we developed an improved promoter part. It will allow for tightly-regulated expression of our recombinases in both prokaryotes and eukaryotes. This construct in particular assayed Vika/Vox recombination | ||
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Revision as of 12:02, 7 October 2017
Vox-Term-Vox Measurement Construct
Vox-Term-Vox construct inserted in BBa_J04450 part. If Vika recombinase is present it will cause recombination between the two Vox sites, excising the terminator and allowing expression of the RFP protein. Therefore, this plasmid can be used as an assay for determining levels of Vika recombinase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 880
Illegal AgeI site found at 992 - 1000COMPATIBLE WITH RFC[1000]