Difference between revisions of "Part:BBa K2406051"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Recombinases are crucial tools for biotechnology. As our designed proofs of concept illustrate, they are excellent tools for dynamic manipulation of gene expression. To accurately test for recombinase function, a simple, reliable assay is required. This is provided by our target site-term-target site constructs. These are inserted between a promoter and an RBS for a fluorescent protein. If the recombinase recognises the target sites, then recombination will occur and the terminator will be excised, leading to expression of the fluorescent reporter. This construct was used to assay Dre/Rox recombination activity and efficiency. | Recombinases are crucial tools for biotechnology. As our designed proofs of concept illustrate, they are excellent tools for dynamic manipulation of gene expression. To accurately test for recombinase function, a simple, reliable assay is required. This is provided by our target site-term-target site constructs. These are inserted between a promoter and an RBS for a fluorescent protein. If the recombinase recognises the target sites, then recombination will occur and the terminator will be excised, leading to expression of the fluorescent reporter. This construct was used to assay Dre/Rox recombination activity and efficiency. | ||
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Revision as of 11:11, 7 October 2017
Rox-Term-Rox Measurement construct.
Rox-Term-Rox construct inserted in BBa_J04450 part. If Dre recombinase is present it will cause recombination between the two rox sites, excising the terminator and allowing expression of the RFP protein. Therefore, this plasmid can be used as an assay for determining levels of Dre recombinase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 876
Illegal AgeI site found at 988 - 1000COMPATIBLE WITH RFC[1000]