Difference between revisions of "Part:BBa K2404003"
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__NOTOC__
 
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<partinfo>BBa_K2404003 short</partinfo>
 
<partinfo>BBa_K2404003 short</partinfo>
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The [https://apps.araport.org/thalemine/report.do?id=1025303 PR2 promotor] is a regulatory sequence from <i>Arabidopsis thaliana</i> that has been shown to [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1996.10061089.x/full respond to salicylic acid].
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This promotor is taken from sequence -642 > -145 upstream of the PR2 translational start point.
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This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.
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This part is has been cloned into the pSB1C3 plasmid using BsmBI.
  
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This is the most 3’ 500bp of an inducible promoter that has been isolated from <i> Arabidopsis thaliana </i>. It responds to salicylic acid. This part is suitable for Golden Gate cloning using type IIS restriction enzymes. This part is contained in the pSB1C3 plasmid.
 
  
 
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Revision as of 11:36, 6 October 2017


PR2 shortened - a salicylic acid-induced promoter The PR2 promotor is a regulatory sequence from Arabidopsis thaliana that has been shown to [http://onlinelibrary.wiley.com/doi/10.1046/j.1365-313X.1996.10061089.x/full respond to salicylic acid].

This promotor is taken from sequence -642 > -145 upstream of the PR2 translational start point.

This part conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] standard and contains the 5' GGAG and 3' AATG sequences for cloning into level 1 plasmids.

This part is has been cloned into the pSB1C3 plasmid using BsmBI.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 607
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 540
    Illegal PstI site found at 582
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 794