Difference between revisions of "Part:BBa K2491000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The catabolic pathway was synthetized as two polycistronic operons with the codon optimized for E.coli expression, eliminating any prefix and suffix restriction sites and any overlapping sequences. In addition, each operon is under the control of its own promoter parts (insert 1 and insert 2) with its own terminator sequence and with RBS sequences inserted between codons. | + | The catabolic pathway was synthetized as two polycistronic operons with the codon optimized for E.coli expression, eliminating any prefix and suffix restriction sites and any overlapping sequences. In addition, each operon is under the control of its own promoter parts (insert 1 and insert 2) with its own terminator sequence and with RBS sequences inserted between codons. |
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===Source=== | ===Source=== | ||
Latest revision as of 20:47, 5 October 2017
Fluorene Degradation Sequence First Part 100
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 403
Illegal BsaI.rc site found at 1515
Design Notes
The catabolic pathway was synthetized as two polycistronic operons with the codon optimized for E.coli expression, eliminating any prefix and suffix restriction sites and any overlapping sequences. In addition, each operon is under the control of its own promoter parts (insert 1 and insert 2) with its own terminator sequence and with RBS sequences inserted between codons.
Source
Synthetic Genes based on Terrabacter sp. DBF63