Difference between revisions of "Part:BBa K2443044"
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Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker.  
 
Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker.  
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Amino acid composition:
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Ala (A)  49 10.3%
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Arg (R)  30   6.3%
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Asn (N)  18   3.8%
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Asp (D)  38   8.0%
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Cys (C)  5   1.1%
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Gln (Q)  16   3.4%
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Glu (E)  34   7.2%
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Gly (G)  37   7.8%
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His (H)  22   4.6%
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Ile (I)  21   4.4%
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Leu (L)  36   7.6%
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Lys (K)  23   4.8%
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Met (M)  21   4.4%
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Phe (F)  19   4.0%
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Pro (P)  15   3.2%
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Ser (S)  21   4.4%
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Thr (T)  18   3.8%
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Trp (W)  7   1.5%
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Tyr (Y)  16   3.4%
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Val (V)  29   6.1%
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Pyl (O)  0   0.0%
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Sec (U)  0   0.0%
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(B)  0   0.0%
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(Z)  0   0.0%
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(X)  0   0.0%
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Total number of negatively charged residues (Asp + Glu): 72
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Total number of positively charged residues (Arg + Lys): 53
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Revision as of 17:52, 5 October 2017


Cys RS

Cysteine tRNA synthetase responsible for attaching cysteine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker.

Amino acid composition: Ala (A) 49 10.3% Arg (R) 30 6.3% Asn (N) 18 3.8% Asp (D) 38 8.0% Cys (C) 5 1.1% Gln (Q) 16 3.4% Glu (E) 34 7.2% Gly (G) 37 7.8% His (H) 22 4.6% Ile (I) 21 4.4% Leu (L) 36 7.6% Lys (K) 23 4.8% Met (M) 21 4.4% Phe (F) 19 4.0% Pro (P) 15 3.2% Ser (S) 21 4.4% Thr (T) 18 3.8% Trp (W) 7 1.5% Tyr (Y) 16 3.4% Val (V) 29 6.1% Pyl (O) 0 0.0% Sec (U) 0 0.0% 

(B)   0	  0.0%
(Z)   0	  0.0%
(X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 72 Total number of positively charged residues (Arg + Lys): 53


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 993
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 859