Difference between revisions of "Part:BBa K2308002:Design"

 
 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
If the up/downstream  sequence in genome is different from the part, the efficiency of homologous recombination will be much lower.
 
If the up/downstream  sequence in genome is different from the part, the efficiency of homologous recombination will be much lower.
 +
There's NO spacer between BBa_K2308008& BBa_K2308003.
  
  

Latest revision as of 07:11, 5 October 2017


sYFP2 knock In for fusion expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 319
    Illegal BamHI site found at 1217
    Illegal XhoI site found at 96
    Illegal XhoI site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 36
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 336
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If the up/downstream sequence in genome is different from the part, the efficiency of homologous recombination will be much lower. There's NO spacer between BBa_K2308008& BBa_K2308003.


Source

The up/downstream sequences are from the genome of Rhodobacter Sphaeroides, and the sYFP2 coding sequence is from Part:BBa_K2308003 .

References