Difference between revisions of "Part:BBa K2308002:Design"
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===Design Notes=== | ===Design Notes=== | ||
If the up/downstream sequence in genome is different from the part, the efficiency of homologous recombination will be much lower. | If the up/downstream sequence in genome is different from the part, the efficiency of homologous recombination will be much lower. | ||
| + | There's NO spacer between BBa_K2308008& BBa_K2308003. | ||
Latest revision as of 07:11, 5 October 2017
sYFP2 knock In for fusion expression
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 319
Illegal BamHI site found at 1217
Illegal XhoI site found at 96
Illegal XhoI site found at 159 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 36
Illegal NgoMIV site found at 54
Illegal NgoMIV site found at 336 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
If the up/downstream sequence in genome is different from the part, the efficiency of homologous recombination will be much lower. There's NO spacer between BBa_K2308008& BBa_K2308003.
Source
The up/downstream sequences are from the genome of Rhodobacter Sphaeroides, and the sYFP2 coding sequence is from Part:BBa_K2308003 .