Difference between revisions of "Part:BBa K2443000"
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Alanine tRNA synthetase responsible for attaching Alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a N-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter (BBa_I719005), RBS (BBa_B0034) and double terminator (BBa_B0015).
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__NOTOC__
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<partinfo>BBa_K2443003 short</partinfo>
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Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in <i>Escherichia coli</i>. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2443003 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K2443003 parameters</partinfo>
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Revision as of 22:42, 4 October 2017


AspRS optimized for expression in E. coli

Alanine tRNA synthetase responsible for attaching alanine onto its tRNA to form an aminoacyl-tRNA. Codon optimized for use in Escherichia coli. Contains a C-terminal histidine tag with a serine glycine linker. Under the regulation of T7 Promoter, RBS and double terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 835
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 752