Difference between revisions of "Part:BBa K2443043:Design"

 
(→‎Source)
Line 16: Line 16:
 
Sequence obtained from:
 
Sequence obtained from:
 
http://www.uniprot.org/uniprot/P21889
 
http://www.uniprot.org/uniprot/P21889
−
His tag terminal:
+
C-terminal His tag chosen because of the following publication:
 
Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M.,  Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. <i>ACS Synth. Biol. 2</i>: 43-52
 
Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M.,  Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. <i>ACS Synth. Biol. 2</i>: 43-52
  
 
===References===
 
===References===

Revision as of 22:26, 4 October 2017


Asp RS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 703


Design Notes

Codon optimized for use in Escherichia coli.



Source

Sequence obtained from: http://www.uniprot.org/uniprot/P21889 C-terminal His tag chosen because of the following publication: Wang H., Huang P., Xu G., Haas W., Marblestone A., Li J., Gygi S., Forster A., Jewett M., Church G. (2012) Multiplexed in Vivo His- Tagging of Enzyme Pathways for in Vitro Single-pot Multienzyme Catalysis. ACS Synth. Biol. 2: 43-52

References